purification and measurement of secretory iga in

Frontiers

For IgA purification the same procedure was followed using 2 μL CaptureSelect IgA Affinity Matrix beads (Thermo Fisher Scientific Breda The Netherlands) For IgG purification either 100 μL of saliva or 2 μL of plasma was added to each well while 100 μL of saliva or 5 μL of plasma was used for IgA purification

Human Fc Receptor

The key role of secretory IgA in neutralizing pathogens in mucosal surfaces is well established Agarwal et al propose that secretory IgA entering the body through breached mucosa engages FCRL3 on regulatory T cells and suppresses their inhibitory function FCRL3 could be therapeutically targeted to modulate regulatory T cell activity

Selective IgA Deficiency

2016-3-30IgA deficiency although research toward purification of human IgA is ongoing However it remains to be seen if replacement of IgA by any route (IV oral or topical) will be beneficial for humans with IgA deficiency in part because IgA in the serum unlike IgG

JP2001503981A

JP2001503981A JP52101598A JP52101598A JP2001503981A JP 2001503981 A JP2001503981 A JP 2001503981A JP 52101598 A JP52101598 A JP 52101598A JP 52101598 A JP52101598 A JP 52101598A JP 2001503981 A JP2001503981 A JP 2001503981A Authority JP Japan Prior art keywords sc secretory antigen antibody iga Prior art date 1996-11-01 Legal status (The legal status is an

Oral Microbial Ecology and the Role of Salivary

SUMMARY In the oral cavity indigenous bacteria are often associated with two major oral diseases caries and periodontal diseases These diseases seem to appear following an inbalance in the oral resident microbiota leading to the emergence of potentially pathogenic bacteria To define the process involved in caries and periodontal diseases it is necessary to understand the ecology of the

Selective IgA Deficiency

Individuals with Selective IgA Deficiency lack IgA but usually have normal amounts of the other types of immunoglobulins Selective IgA Deficiency is relatively common in Caucasians Many affected people have no illness as a result Others may develop a variety of significant clinical problems Definition of Selective IgA Deficiency Selective IgA Deficiency is defined as a

Purification and Characterisation of Badger IgA and Its

To develop an IgA assay we purified secretory IgA from badger bile identifying secretory component (SC) heavy chain (HC) and light chain (LC) at 66 46 and 27 Kda respectively on the basis of size comparison with other species Monoclonal antibodies (mAbs) were generated to purified IgA We selected two for ELISA development

US20140359902A1

US20140359902A1 US14/365 934 US201214365934A US2014359902A1 US 20140359902 A1 US20140359902 A1 US 20140359902A1 US 201214365934 A US201214365934 A US 201214365934A US 2014359902 A1 US2014359902 A1 US 2014359902A1 Authority US United States Prior art keywords duckweed chain antibody method according siga Prior art date 2011-12-16 Legal status (The legal

US20140359902A1

US20140359902A1 US14/365 934 US201214365934A US2014359902A1 US 20140359902 A1 US20140359902 A1 US 20140359902A1 US 201214365934 A US201214365934 A US 201214365934A US 2014359902 A1 US2014359902 A1 US 2014359902A1 Authority US United States Prior art keywords duckweed chain antibody method according siga Prior art date 2011-12-16 Legal status (The legal

The function of immunoglobulin A in immunity

Secretory and serum IgA are therefore molecules with different biochemical and immunochemical properties produced by cells with different organ distributions Different methods of immunization can induce serum or secretory IgA responses or a combination of both It appears that in mice secretory IgA in the gut comes from two sources 23 24

bs

Host: Rabbit Target Protein: human secretory IgA Clonality: Polyclonal Isotype: IgG Entrez Gene: 4504 Swiss Prot: P25713 Source: Purified human sIgA Purification: Purified by Protein A Storage: Aqueous buffered solution containing 1% BSA 50% glycerol and 0 09% sodium azide Store at 4C for 12 months Background: Binds heavy metals Contains three zinc and three copper atoms per polypeptide

Purified Human Secretory IgA MP Biomedicals

Product is the lyophilized powder of human secretory IgA and buffer salts Proteins are used in routine laboratory procedures such as binding enzymes or coupling peptides to carrier proteins These kits mixture solutions and collagen matrices fulfill a myriad of essential laboratory functions for developing relationships between proteins and other cellular components

Frontiers

For IgA purification the same procedure was followed using 2 μL CaptureSelect IgA Affinity Matrix beads (Thermo Fisher Scientific Breda The Netherlands) For IgG purification either 100 μL of saliva or 2 μL of plasma was added to each well while 100 μL of saliva or 5 μL of plasma was used for IgA purification

Frontiers

For IgA purification the same procedure was followed using 2 μL CaptureSelect IgA Affinity Matrix beads (Thermo Fisher Scientific Breda The Netherlands) For IgG purification either 100 μL of saliva or 2 μL of plasma was added to each well while 100 μL of saliva or 5 μL of plasma was used for IgA purification

Human IgA

The IgA purified from human saliva using our system exhibited a peak of the dimeric secretory form of IgA with a molecular mass of 400 000 (supplemental Fig S2) These results indicate that our peptide is suitable for the purification of both subclasses/subtypes of IgA from various body fluids in the same way as bacterial IgA-binding proteins

Plant

A key module secretory component (SC) was efficiently expressed in Arabidopsis thaliana The plant-based SC and immunoglobulin A of animal or plant origin formed secretory IgA that maintains antigen-binding activity Plant expression systems are suitable for scalable and cost-effective production of biologics Secretory immunoglobulin A (SIgA) will be useful as a therapeutic antibody against

A pathogenic role for secretory IgA in IgA

IgA nephropathy (IgAN) is characterized by deposits of IgA in the renal mesangium It is thought that deposits of IgA mainly involve high molecular weight (HMW) IgA1 However there is limited information on the exact composition of HMW IgA in these deposits In this study we investigated the presence of secretory IgA (SIgA) in human serum and in the glomerular deposits of a patient with IgAN

Antibody Purification Methods

The lectin is a glycoprotein of approximately 40kDa composed of four identical subunits Jacalin immobilized on supports such as agarose has been useful for the purification of human serum or secretory IgA1 The affinity ligand makes it possible to purify or remove IgA from the more abundant IgG and IgM in human serum or colostrum (6)

Affinity chromatographic purification of human

Immobilized streptococcal IgA‐binding peptide (Sap) a 50‐residue synthetic peptide derived from protein Sir22 was used for single‐step purification of secretory IgA from human saliva with 45% recovery and highly purified serum IgA was achieved with over 99% recovery after passage through a Sap column 28 This synthetic peptide for

Structural and Functional Consequences of Cleavage

The cleavage of human serum monomeric immunoglobulin A1 (IgA1) and human secretory IgA1 (S-IgA1) by IgA1 proteinase of Neisseria meningitidis and cleavage by the proteinase from Proteus mirabilis have been compared For serum IgA1 both proteinases cleaved only the α chain N meningitidis proteinase cleaved only in the hinge P mirabilis proteinase sequentially removed the tailpiece the

Purification and Characterization of Human Serum

Abstract Human immunoglobulin A (IgA) is found in serum and secretions in several different forms () Serum IgA is produced by the bone marrow and is pre-dominately monomeric (M r = 160 kDa) Most of the IgA in secretions (sIgA) is polymeric predominantly dimeric comprising two monomer subunits linked together by intersubunit disulfide bonds and a cysteine-rich polypeptide termed J-chain (M

BioVision

BioVision develops and offers a wide variety of products including assay kits antibodies recombinant proteins enzymes and other innovative research tools for studying Apoptosis Metabolism Cell Proliferation Cellular Stress Cell Damage and Repair Diabetes Obesity and Metabolic Syndrome Stem Cell Biology Gene Regulation Signal Transduction etc BioVisions products are currently

A pathogenic role for secretory IgA in IgA

IgA nephropathy (IgAN) is characterized by deposits of IgA in the renal mesangium It is thought that deposits of IgA mainly involve high molecular weight (HMW) IgA1 However there is limited information on the exact composition of HMW IgA in these deposits In this study we investigated the presence of secretory IgA (SIgA) in human serum and in the glomerular deposits of a patient with IgAN

Secretory IgA mediates retrotranscytosis of intact

Secretory IgA mediates retrotranscytosis of intact gliadin peptides via the transferrin receptor in celiac disease Measurement of IgA and IgA-containing complexes in serum and duodenal secretions nonspecific IgA1 (mIgA dIgA and pIgA at a final concentration of 50 μg/ml respectively prepared as described in Purification of IgA

Expression purification and biochemical

1999-7-15Parr EL Bozzola JJ Parr Purification and measurement of secretory IgA in mouse milk J Immunol Methods 1995 Mar 27 180 (2):147–157 [Google Scholar] Pierre PG Havaux XB Langendries A Courtoy PJ Goto K Maldague P Vaerman JP Mouse secretory component Adv Exp Med Biol 1995 371A:629–632 [Google Scholar]